Figure 5.

The expression of TSLP and IL-23/IL-17 in P. aeruginosa–infected mouse corneas with or without TSLPR neutralization. Mouse corneas were subconjunctivally injected TSLPR neutralizing antibody at −4 hours. At 0 hours, the corneas were incubated with 1.0 × 10⁴ CFU of P. aeruginosa. (A) Micrographs and clinical scores of the control (IgG) and TSLPR neutralizing antibody treated corneas at 1 dpi. (B) Corneas (3 in each group) were excised from the enucleated eyes at 1 dpi and subjected to Real-time PCR analysis of TSLP, IL-23, IL-17A, and IL-17C. The results are presented as fold increase with the levels of naïve corneas set as 1. The Figure is representative of two independent experiments, each with five corneas. *P < 0.05. **P < 0.01 (1-way ANOVA, with Bonferroni correction).