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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Glycobiology. 2018 Jul 1;28(7):474–481. doi: 10.1093/glycob/cwy045

Figure 2. RP-HPLC and MALDI-TOF-MS analyses of GAG-like O-glycans from Oesophagostomum dentatum.

Figure 2

(A and B) After hydrazinolysis and pyridylamination, glycans from male and female O. dentatum were separated on an RP-amide column; the zoom of the chromatograms from females (A) and males (B) focuses on the region in which GAG-like O-glycans elute. (C-H) Positive mode MS of male fractions I-VI is compatible with the basic sequence of [HexNAcn,n-1-GlcAβ3n]-Galβ3-Galβ4-Xyl-PA as well as unprecedented core decoration with phosphorylcholine (PC) and/or galactose. Proposed structures (all [M+H]+) were confirmed by MS/MS as well as by chemical or enzymatic digestions. Calibration is in terms of glucose units (g.u.). Elution positions of major N-glycans between 5-24 min are annotated (Jimenez-Castells et al., 2017). For the ODS-C18 RP-HPLC and HIAX NP-HPLC chromatograms of O. dentatum GAG-like O-glycans, refer to Supplementary Figure 3.