Table 1.
High expression of gene ontologies in podocytes after exposure to IgG from systemic lupus erythematosus patients*
| Ontology | Term | No. of changed genes |
Total no. of genes |
Z score | P |
|---|---|---|---|---|---|
| Biologic process | Cellular component assembly involved in morphogenesis |
10 (0) | 257 (0) | 2.787 | 0.01313 |
| Biologic process | Regulation of T cell activation† | 12 (0) | 397 (8) | 2.112 | 0.04711 |
| Biologic process | Actin filament–based process | 25 (0) | 945 (1) | 2.353 | 0.02711 |
| Cellular component | Cell–substrate adherens junction | 10 (0) | 290 (12) | 2.383 | 0.03349 |
| Cellular component | Focal adhesion | 10 (10) | 282 (282) | 2.478 | 0.03051 |
| Cellular component | Axon part | 10 (2) | 281 (37) | 2.49 | 0.03018 |
| Cellular component | I band | 10 (3) | 185 (28) | 3.986 | 0.00146 |
| Cellular component | Cell–substrate junction | 11 (2) | 305 (12) | 2.66 | 0.02082 |
| Cellular component | Early endosome | 12 (10) | 362 (256) | 2.463 | 0.02293 |
| Cellular component | Contractile fiber part | 13 (0) | 290 (0) | 3.761 | 0.00156 |
| Cellular component | Sarcomere | 13 (1) | 260 (33) | 4.214 | 0.000606 |
| Cellular component | Contractile fiber | 14 (0) | 335 (11) | 3.608 | 0.00196 |
| Cellular component | Myofibril | 14 (1) | 318 (55) | 3.827 | 0.00123 |
| Cellular component | Endosome membrane | 16 (9) | 525 (282) | 2.487 | 0.02362 |
| Cellular component | Endosomal part | 17 (0) | 541 (0) | 2.697 | 0.0161 |
| Cellular component | Basolateral plasma membrane | 17 (5) | 585 (258) | 2.357 | 0.032 |
| Cellular component | Endosome | 27 (14) | 1,040 (587) | 2.361 | 0.02568 |
| Cellular component | Z disc | 9 (9) | 171 (171) | 3.687 | 0.00294 |
| Molecular function | Cytokine receptor binding | 10 (0) | 303 (11) | 2.234 | 0.03951 |
| Molecular function | Magnesium ion binding | 12 (12) | 368 (368) | 2.403 | 0.03678 |
*
Specific ontologies with the highest Z scores were selected. Criteria for inclusion in the table were an ontology containing ≥9 genes and having a Z score of >2.0. Numbers in parentheses are the numerical value considered in the hierarchical structure of each gene ontology term.
†
Term selected because the genes with the highest changes included HLA–DQB1, IL10, PTPN22, CAMK4, CD80, and CD86, all of which have also been known to be linked to the pathogenesis of lupus.