Fig 4. Measurement reproducibility for kinetic parameterization.

In panels A-C, kinetic curves created by images series of varying exposure times (v_i,e) are compared to the more robust v_i,·. The data presented is 144 peptides for all 12 samples discussed in Methods. Panel A is a 10 ms exposure time and has a Pearson's r = 0.9981 and a Spearman's ρ = 0.9097. Panel B is a 50 ms exposure time and has a Pearson's r = 0.9989 and a Spearman's ρ = 0.9920. Panel C is a 200 ms exposure time and has a Pearson's r = 0.9807 and a Spearman's ρ = 0.9995. Panels D compares ls(v_i,50 (s), v_i,50,b(b)) to ls(v_i,· (s), v_i,·,b(b)) and has a Pearson's r = 0.9887 and a Spearman's ρ = 0.9279. Panels E compares $ls\left(v_{i,50}\right(s),v_{i,50,\widehat{b}}(\widehat{b}\left)\right)$ to $ls\left(v_{i,·}\right(s),v_{i,·,\widehat{b}}(\widehat{b}\left)\right)$ and has a Pearson's r = 0.9941; Spearman's ρ = 0.9681. These figures and all similar variations can be further investigated and explored at http://bit.kinomecore.com/?fig4a for b and at http://bit.kinomecore.com/?fig4b for $\widehat{b}$.