Fig 4. Time-dependence of the antiviral activity of the endocytic inhibitors.

Cells were treated with 50 mM ammonium chloride (A), 20 μM chlorpromazine (B) or 160 μM dynasore (C) before infection with DENV-2 (pretreatment) or after 2 h of infection with DENV-2 (post internalization) at a m.o.i. of 5 PFU/cell. At 48 h p.i. the extracellular virus yields were determined by plaque formation in Vero cells and the results are expressed as % of virus yield in treated infected cells with respect to a control of infected cells without drug treatment. Each bar is the mean of three independent experiments ± SEM.