. 2018 Aug 9;12(8):e0006705. doi: 10.1371/journal.pntd.0006705

Table 2. Sequence-specific primers^* for selected CR1 polymorphisms.

FORWARD PRIMERS				REVERSE PRIMERS
	SNP	Region	5’→3’ Sequence	SNP	Region	5’→3’ Sequence	bp
Simple PCR	rs6656401	Intron 4	CTCTGTCTCCATCTTCTCA	Generic Primer	Intron 4	CATAGTTGTAGTTGGGGATTG	257
Simple PCR	rs6656401	Intron 4	CTCTGTCTCCATCTTCTCG	Generic Primer	Intron 4	CATAGTTGTAGTTGGGGATTG	257
Multi.2	rs3849266	Intron 21	CTGATGGCTTGGGGTAT	rs2274567	Exon 22	CTCAATCTGCATTGATCCAC	667
Multi.2	rs3849266	Intron 21	CTGATGGCTTGGGGTAC	rs2274567	Exon 22	CTCAATCTGCATTGATCCAT	667
Multi.2	rs4844610	Intron 37	CTACACAAAACAGCCTTGTA	rs12034383	Intron 37	AGATGTCCATGCCTTAAC	1080
Multi.2	rs4844610	Intron 37	CTACACAAAACAGCCTTGTC	rs12034383	Intron 37	AGATGTCCATGCCTTAAT	1080
Multi.1	rs3737002	Exon 26	CCATTTGCCAGTCCTAC	rs11118131	Intron 26	CAAGAAGAAGGGGTGATG	457
Multi.1	rs3737002	Exon 26	CCATTTGCCAGTCCTAT	rs11118131	Intron 26	CAAGAAGAAGGGGTGATA	457
Multi.1	rs11118167	Intron 28	GCCAATATGTGAATATTATTATCTTAT	rs17047660	Exon 29	TTCTGGAGCTGTGCATTT	746
Multi.1	rs11118167	Intron 28	GCCAATATGTGAATATTATTATCTTAC	rs17047660	Exon 29	TTCTGGAGCTGTGCATTC	746
Control Primers
Multi. 2 / Simple PCR		HGH f	TGCCTTCCCAACCATTCCCTTA		HGH r	CCACTCACGGATTTCTGTTGTGTTTC	431
Multi. 1		HLA-E f	CGGGACTGACTAAGGGGCGG		HLA-E r	GTAGCCCTGTGGACCCTCTTAC	324

* excepting the generic and control primers. In bold: variant nucleotide.

Multi: multiplex; SNP: single nucleotide polymorphism; bp: base pairs; f: forward; r: reverse; HGH: Human Growth Hormone; HLA-E: Human Leukocyte Antigen E

Table 2. Sequence-specific primers* for selected CR1 polymorphisms.

Table 2. Sequence-specific primers^* for selected CR1 polymorphisms.