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. 2018 Aug 9;14(8):e1007543. doi: 10.1371/journal.pgen.1007543

Fig 6. Genetic control of FB formation.

Fig 6

(A) Schematics of AvrII digest of Chr III (OF) in IR-1000 and its DSB-induced derivatives including: CF, ID, FB. (B) Analysis of ID and FB formation in IR-1000 containing one of the following deletions: rad1Δ, rad10Δ, saw1Δ, slx4Δ, rad14Δ, slx1Δ, mus81Δ, yen1Δ, rad2Δ, rad1Δslx1Δ, rad1Δmus81Δ, rad1Δyen1Δ or rad1Δrad2Δ using AvrII digest of DNA, gel electrophoresis and hybridization with probe P-1 (indicated in Fig 6A by black box). (C) The role of RAD52 and RAD51 in FB formation in IR-1000 derivatives analyzed by AvrII digest followed by hybridization with probe P-1. (D) The schematics of AvrII digest of Chr III in IR-12 strain (OF) and its DSB-induced derivatives: CF, ID, FB. (E). Analysis of ID and FB formation in sae2Δ, sae2Δrad52Δ, and sae2Δrad51Δ derivatives of IR-12 following AvrII digestion and hybridization with probe P-1.