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. 2018 Aug 9;14(8):e1007543. doi: 10.1371/journal.pgen.1007543

Fig 7. IRs channel DSB repair into BIR and chromosome loss.

Fig 7

(A) (i) Schematics of diploid experimental strain where a DSB is induced by a galactose-inducible HO-endonuclease at MATa locus on the chromosome III (top blue). The MATα-inc chromosome (bottom grey) is resistant to cutting by HO. The HML and HMR in the MATa containing chromosome are replaced by ADE1. (ii) Gene conversion outcome—Ade+ Thr+ Ura+. (iii) Break induced replication outcome: Ade+ Thr- Ura+. (iv) Chromosome loss outcome: Ade- Thr- Ura+. (B) The frequency of gene conversion (including gene conversion with and without crossing-over; (GC)), BIR and chromosome loss (Loss) following DSB induction in diploid strains with and without IRs. Results of 3 to 10 experiments were used to calculate the median frequencies and the ranges of the medians [in brackets] of GC, BIR and Loss outcomes. Asterisks indicate statistically significant (P <0.01) changes compared to the No-IR strain.