Figure 4.

The Treatment Effects of Calotroposide A Compound on Caspase-8 Expression on WiDr Cells. WiDr Cells with (5x10⁴) density were incubated on coverslips in 12 well plates for 24 hours to adapt. Then, Calotroposid A compound was treated on IC₅₀ 17.23 μg/ml, and then incubated for another 15 hours while caspase-8 staining was conducted using immunocytochemistry method in accordance with the research procedure. (A) cell control without caspase-8 antibody, (B) cells with Calotroposide A compound treatment with anti-caspase-8 antibody indicated an increase on caspase-8 protein expression as seen from the cytosol brown stain. The arrow (b) indicates caspase-8 expression. The microscope used was a light microscope with 400x magnification.