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Antibodies and conditions employed.
| Target | Dilution | Source |
|---|---|---|
| TARDBP | 1:1000 in TBS-T 0.05% | Proteintech, 10782–2-AP |
| ATG4B | 1:250 in TBS-T 0.05% | Sigma-Aldrich, A2981 |
| SQSTM1 | 1:1000 in TBS-T 0.05% (Western blot); 1:100 in PBS (immunocytofluorescence) | Cell Signalling Technology, 5114 |
| Secondary anti-rabbit, HRP conjugate | 1:50000 in TBS-T 0.05% | Thermo Fisher Scientific, 31460 |
| Secondary anti-rabbit, Alexa Fluor® 546 conjugate | 1:800 in PBS | Thermo Fisher Scientific, A11010 |
