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. 2018 Jul 20;14(8):1376–1397. doi: 10.1080/15548627.2018.1476809

Figure 9.

Figure 9.

The autophagy and HCK signaling of ESCs are involved in regulating the PTGS2 level in NK cells by MIR1185-1-3p. . (a) The NK function-related genes in NK cells cocultured with Ctrl-ESC and 3-MA-ESC by Human Gene 2.0 ST Array. (b) The intersection analysis between differential genes in NK cells (coculture with Ctrl-ESC or 3-MA-ESC) and predicted target genes (i.e. EGR2, EREG and PTGS2) of MIR1185-1-3p. (c) The bioinformatics analysis (KEGG database-derived Signal net) about the relationship between PTGS2 (COX-2), FCGR3, CXCL8 and IL23A. (d,e) The relative luciferase activity of PTGS2 in HEK-293T cells was analyzed by a dual luciferase reporter assay, after cotransfection with plasmids (NC, MIR1185-1-3p mimics, MIR1185-1-3p inhibitor) and luciferase reporter plasmids (PTGS2-Luc [WT], PTGS2 mutation-Luc [MUT]) (Student t test). (f) FCM analysis of PTGS2 level in NC NK cells (n = 6) and MIR1185-1-3p + NK cells (n = 6) after coculture with Ctrl-ESC or siHCK-ESC (one-way ANOVA). (g) FCM analysis of PTGS2 level in NC NK cells (n = 6) and MIR1185-1-3p +NK cells (n = 6) after coculture with NC ESC or 3-MA-ESC (one-way ANOVA). (h) FCM analysis of PTGS2 level in NK cells of PF from WT (n = 9 mice/group) and hck−/- (n = 10 mice/group) EMS mice by FCM (Student t test). MIR1185-1-3p mimics, HsMIR1185-1-3p mimics; MIR1185-1-3p inhibitor, HsMIR1185-1-3p inhibitor. Data are expressed as the mean± SEM. **P < 0.01, ***P < 0.001 and ****P < 0.0001.