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. 2018 Jun 23;17(8):974–984. doi: 10.1080/15384101.2018.1464834

Figure 7.

Figure 7.

LINC00152 regulates the expression of the miR-193a/b-3p target gene, CCND1. (a) Bioinformatic analysis using TargetScan identified CCND1 as a candidate target gene of miR-193a/b-3p. (b) The 3ʹ-UTR of CCND1 was cloned into the pmirGLO vector and co-transfected into Hep3B cells along with either the miR-193a-3p, or miR-193b-3p mimic, or with pcDNA3.1-LINC00152. Luciferase activities were detected using the dual-luciferase assay. Cells co-transfected with pmirGLO-CCND1 and either miR-193a-3p or miR-193b-3p showed a decrease in luciferase activity, while cells co-transfected with pcDNA3.1-LINC00152 and pmirGLO-CCND1 showed an increase in luciferase activity. Cells co-transfected with pcDNA3.1-LINC00152, pmirGLO-CCND1 and either miR-193a-3p or miR-193b-3p showed no marked change (**p < 0.01, NS, not significant). (c) The expression of CCND1 mRNA was analysed in HCC cells following the overexpression of LINC00152 and either miR-193a-3p or miR-193b-3p. CCND1 mRNA expression decreased in cells transfected with either miR-193a-3p or miR-193b-3p compared with negative control cells, and CCND1 mRNA expression increased in cells transfected with pcDNA3.1-LINC00152. Cells co-transfected with pcDNA3.1-LINC00152 and either miR-193a-3p or miR-193b-3p showed a marked decrease in CCND1 mRNA expression compared with cells pcDNA3.1-LINC00152 alone (**p < 0.01, NS, not significant). (d) The expression of CCND1 protein was analysed in HCC cells following the overexpression of LINC00152 and either miR-193a-3p or miR-193b-3p. The expression level of the CCND1 protein was consistent with that of the CCND1 mRNA. (e) MiR-193a/b-3p abrogated the ability of LINC00152 to induce cell proliferation in Hep3B cells (*p < 0.05, NS, not significant).