Table 1.
Steady-state kinetic parameters for editing of non-cognate aa by various aaRSs.
| aaRS | Non-cognate aa | Km (mM) | kcat (min−1) |
|---|---|---|---|
| Pf-AlaRS | L-Gly | 18.7 ± 1.3 | 5.2 ± 1.3 |
| L-Ser | 97.1 ± 14.8 | 2.7 ± 0.1 | |
| Mt-AlaRS | L-Gly | 3.9 ± 0.3 | 1.8 ± 0.3 |
| Mt-ValRS | L-Thr | 1.6 ± 0.1 | 3.2 ± 0.1 |
| Mt-IleRS | L-Val | 0.1 ± 0.02 | 0.9 ± 0.02 |
| Ec-IleRS | L-Val | 1.86 ± 0.5 | 39.6 ± 1.0 |
| Ec-ThrRS | L-Ser | 22.2 ± 1.0 | 14.0 ± 1.0 |
| Ec-LysRS | L-Hc | 45.0 ± 6.4 | 15.8 ± 6.4 |
a
Hc, homocysteine;
b
The active concentration of enzyme was not determined and the k cat values reported in this table are apparent. The total concentration of aaRS in the assay (see Materials and Methods) was used to derive apparent k cat values.