Table 2.
aaRS compatibility chart for multi-enzyme HTS assay.
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a
Light gray boxes indicate aaRSs that can be assayed simultaneously using any of the non-cognate aa for each enzyme. Medium gray boxes indicate combinations of aaRSs that cannot be assayed simultaneously, if the cognate aa (indicated in the box) of either aaRS is included in the assay. Black boxes indicate a mixture, containing SerRS and ThrRS, that is not amenable to simultaneous screening. Non-cognate aa for editing reactions are from reference [40]. ncaa, non-cognate aa; αA, α-aminobutyrate; Hc, homocysteine; γL, γ-hydroxyleucine; nV, norvaline; nL, norleucine; 4P, 4-hydroxyproline; Hs, homoserine; Or, ornithine; Sx, serine hydroxamate.
b
Class II LysRS