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Resistance of t6A deficient yeast strains to PrrC toxins. A. The tcs3Δ is resistant to PrrCEc while mitochondrial tcs4Δ is not. B. tcs8Δ is resistant to both PrrCEc and PrrCSm, while the PrrCEc K46A variant has no effect on growth. Cells were grown in synthetic minimal media containing agar and leucine dropout supplement (SD-Leu). Galactose (2% w/v) and raffinose (1% w/v) were added where necessary (SD-Leu Gal/Raf). Strains were incubated at 30°C for ∼72 hours.
