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. 2017 Sep 13;15(4-5):508–517. doi: 10.1080/15476286.2017.1353861

Figure 4.

Figure 4.

Detection of t6A in S. mutans wild-type and mutant strains. A. PHAt6A with S. mutans ΔtsaE. B. Mass spec analysis of t6A modification in tRNAIle GAU showing no t6A in ΔtsaC and ΔtsaB but trace amounts are detected in ΔtsaE. C. PHAt6A with t6A deficient strains ΔtsaC, ΔtsaB, ΔtsaE using oligonucleotides specific to tRNAIle GAU. D. PHAt6A with wild type UA159 and ΔtsaE using probes for tRNAiniMet CAU.