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. 2018 Feb 1;15(4-5):635–648. doi: 10.1080/15476286.2017.1356563

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — Overexpression of tRNAs enhanced the cell proliferation. (A) Relative levels (percentage) of cell number, thymidine incorporation, and methionine incorporation after overexpression of each tRNA were compared with those in EV (empty vector)-transfected control. Values are presented as means ± standard deviations (n = 3). *, P < 0.05; ***, P < 0.001. (B) Change in cell cycle after tRNA transfection was compared with that in control. (C) Real-time proliferation of HEK 293T cells transfected with different tRNAs was monitored under different culture conditions. Values are presented as means ± standard errors (n = 9). Values for tRNA^Leu _CAG and negative control (NC) are repeated in all the graphs for the comparison. Cell viability was calculated as the relative percentage of confluency. CM, complete media; SF, serum free; AA, amino acids.