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. 2018 Feb 1;15(4-5):635–648. doi: 10.1080/15476286.2017.1356563

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 4. — RSK1 and MSK2 were the 90-kDa RPS6K phosphorylated by tRNA^Leu _CAG overexpression. (A) Sequence alignment of 8 different RPS6 family kinases. The hydrophobic motif and representative phosphorylation sites are shown in gray and indicated by arrows, respectively. The 502 aa and 525 aa sizes in S6K1 indicate p70 S6K and p85 S6K, respectively. (B and C) Effect of tRNA overexpression on the ERK phosphorylation (B) and qRT-PCR analysis to detect tRNA overexpression (C). Values are presented as means ± standard deviations (n = 3). (D) The effect of ERK (U0126), p38 MAPK (SB203580), and JNK (SP600125) inhibitors on the tRNA^Leu _CAG-mediated p90 RPS6K phosphorylation. (E-G) Western blot analysis for the phosphorylation of RPS6K after overexpression of tRNA^Leu and the different RPS6 family kinases (E), after siRNA transfection specific to p85 S6K and RSK1 (F) as well as RSK2 and MSK2 (G).