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. 2017 Nov 3;15(4-5):528–536. doi: 10.1080/15476286.2017.1377878

Figure 4.

Figure 4.

Intron-containing tRNA lacks Queuosine. A. APB-gel/Northern hybridization was performed on total RNA collected from TbTrl1 RNAi inlduced (+) and uninduced (-) cells. The arrows indicate spliced tRNA and intron-containing tRNA generated by the RNAi knockdown of TbTrl1. Shifted Q-containing (+Q) bands and non-Q-containing-bands (-Q) are as indicated. Samples were treated with sodium periodate to serve as a negative Q control (OX). The experiment was performed with a probe specific for the 3′ exon of tRNATyr. B. The same membrane as in (A) was probed with an intron-specific probe to assess whether intron-containing tRNA contained Q. The higher band located above the intron-containing tRNATyr band is not likely related to Q, as it is also found in the oxidized control lanes. C. Detection of Q in other potential Q-containing tRNAs (tRNAAsp,-Asn and-His) using the samples as in (A). As before, the Q-containing band (+Q) appears as a shifted band as indicated. D. The membrane was further hybridized with a probe specific for tRNAGlu serving as a loading and non-Q containing tRNA control.