Table 2.

Apparent rates and amplitudes of fMet-Val formation in various 70S complexes.

Factors	P-site tRNA	Start codon	kapp, s−1	A
Absent	fMet-tRNAfMet2	AUG	0.99 ± 0.21	0.21 ± 0.02
	fMet-tRNAfMet2	CUG	0.74 ± 0.10	0.087 ± 0.004
	fMet-tRNAfMet2M1	AUG	0.78 ± 0.17	0.20 ± 0.02
	fMet-tRNAfMet2M1	CUG	0.014 ± 0.008	0.028 ± 0.004
Present	fMet-tRNAfMet2	AUG	0.61 ± 0.07	0.54 ± 0.02
	fMet-tRNAfMet2	CUG	0.71 ± 0.05	0.52 ± 0.01
	fMet-tRNAfMet2M1	AUG	0.74 ± 0.12	0.47 ± 0.03
	fMet-tRNAfMet2M1	CUG	0.80 ± 0.05	0.40 ± 0.01

Reported values and their standard errors derive from the curve fits shown in Fig. 6.