Figure 7.

Trehalose promotes TFEB nuclear translocation and activation of the TFEB transcriptional network in vivo. (a) Immunofluorescence of Purkinje cells showing that TFEB was translocated to the nucleus upon trehalose treatment in both WT and naglu^−/- mice. (b) Quantification of the number of cells with TFEB in the nucleus in the indicated genotypes and conditions. (c–d) RT-qPCR analysis of mRNAs extracted from WT (c) or KO (d) brain homogenates showing increased transcriptional levels of TFEB target genes of the autophagy-lysosomal system upon trehalose treatment (n = 3 per group). Data are means ± SE. **p ≤ 0.01. ns, not significant. Scale bar: 30 μm.