Figure 3.
Rb1cc1 deletion diminishes the oxidative respiratory capacity of BPK mammary tumors. (a) Representative OCR plot of BPK primary cells with Rb1cc1F/+ or rb1cc1F/F alleles measured using a Seahorse XF24 extracellular flux analyzer. Arrows mark the injections of respective inhibitors at specific time points. (O, oligomycin A; F, FCCP; R/A, rotenone-antimycin). Quantification of (b) basal, (c) maximal respiration, (d) ATP production, (e) spare capacity, (f) non-mitochondrial and (g) proton leak OCR measurements of BPK primary cells with Rb1cc1F/+ or rb1cc1F/F alleles (n = 4, from independent tumor samples each). Statistical significance was determined by two-tailed t-test, * denotes p ≤ 0.05.