Figure 2.

PTEN-4A Preferentially Localizes to the Nucleus. (A) Total nuclear proteins isolated from H1299 cells stably expressing Flag-tagged PTEN-WT and PTEN-4A revealed that PTEN-4A preferentially localized to the nucleus as compared to PTEN-WT, as examined by immunoblotting with Flag antibodies. (B) Lamin B1, an exclusively nuclear protein, was used as a loading control and to normalize densitometric values obtained for Flag-tagged PTEN-WT and PTEN-4A protein levels in the nucleus. Levels of PTEN-4A was approximately 2.5-fold higher in the nucleus than PTEN-WT. (C) Preferential nuclear localization for PTEN-4A detected by immunofluorescence signals of GFP-tagged PTEN-WT and GFP-tagged PTEN-4A protein expression in 293T cells. Expression of H2B mCherry stained the nucleus red. Data are derived from three independent experiments ± S.E. (n = 3, *p<0.05).