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. 2018 May 31;17(8):947–962. doi: 10.1080/15384101.2017.1388970

Figure 4.

Figure 4.

The PTEN C2 Domain is Required for E2F1-mediated Transcriptional Suppression. (A) The PTEN deletion mutants, PTEN 1–353, PBM-PD (AA 1–185) and C2 domain (C2D, AA 190–350) are stably expressed in transfection assays. (B) Expression of PTEN-4A, PTEN 1–353 and the PTEN C2 domain alone suppressed E2F1-mediated transcription as assessed from the E2F-Luc reporter activity (lanes 2, 3 and 5). However, the PTEN phosphatase domain alone (PBM-PD), which lacks the C2 domain, failed to suppress transcriptional activity (lane 4). These results indicate that the PTEN C2 domain is required for E2F1-mediated transcriptional suppression. (C) Expression of GFP-tagged C2 domain (C2D) as detected by immunofluorescence demonstrate that the PTEN C2D localize to the nucleus (yellow fluorescence). The nucleus is stained red upon expression of histone-H2B mCherry. Nuclear co-localization of GFP-tagged-C2D and histone-H2B mCherry is depicted by yellow fluorescence.