Video 1. Behavior of four flies in response to an ACV 10% pulse.

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DOI: 10.7554/eLife.37815.007

The time of the odor stimulus is signaled by the green dot appearing at the top of the image. Flies start to move upwind shortly after the start of the stimulus (partly due to the time it takes for the odor front to reach their respective positions), and they stop advancing upwind after the odor is gone and engage in a more localized search behavior. Air and odor move from the top of the image towards the bottom at 11.9 cm/s.