Key resources table.
| Reagent type or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-Pan-QKI | Millipore | MABN624 | |
| Antibody | anti-QKI | Bethyl | A300-183A RRID:AB_2173160 |
|
| Antibody | anti-RBFOX1 | Sigma | SAB2100002 RRID:AB_10600323 |
|
| Antibody | anti-MBNL1 | Santa-Cruz | sc-47740 RRID:AB_784435 |
|
| Antibody | anti-MBNL2 | Santa-Cruz | sc-136167 RRID:AB_2140469 |
|
| Antibody | anti-CELF4(Brunol4) | Santa-Cruz | sc-398292 | |
| Antibody | anti-RBFOX2 (RBM9) | Bethyl | A300-864A RRID:AB_609476 |
|
| Antibody | anti-FLNB | Cell signaling | 12979S | |
| Antibody | anti-SNAI1 | Cell signaling | 3879S RRID:AB_2255011 |
|
| Antibody | anti-Vinculin | Cell signaling | 4650S RRID:AB_10559207 |
|
| Antibody | anti-V5 | Cell signaling | 13202 | |
| Antibody | anti-V5 (for eCLIP) | Life Technologies |
R96025 RRID:AB_159313 |
|
| Antibody | anti-CD44-PE-Cy7 | Affymetrix | 25-0441-81 RRID:AB_469622 |
|
| Chemical compound, drug |
siRNA NS #1 | GE Dharmacon | D-001810–01- 05 | |
| Chemical compound, drug |
siFLNB-L1 (3019) | GE Dharmacon | CTM-237650/ LJBYN-000019 |
|
| Chemical compound, drug |
siFLNB-L2 (3021) | GE Dharmacon | CTM-237646/ LJBYN-000021 |
|
| Chemical compound, drug |
siFLNB-S1 (2923) | GE Dharmacon | CTM-237648/ LJBYN-000023 |
|
| Chemical compound, drug | siFLNB-S2 (2925) | GE Dharmacon | CTM-237649/ LJBYN-000025 |
|
| Chemical compound, drug |
siFOXC1 smart pool of 4 siRNAs |
GE Dharmacon | M-009318-01-0005 | |
| Chemical compound, drug |
siFLNB-U1 (targeting UTR region of FLNB) |
GE Dharmacon | CTM-206669 | |
| Chemical compound, drug |
siFLNB-U2 (targeting UTR region of FLNB) |
GE Dharmacon | CTM-206671 | |
| Chemical compound, drug |
Lipofectamine RNAiMAX Transfection Reagent |
thermofisher | 13778150 | |
| Commercial assay or kit |
QIAamp DNA Mini Kit | Qiagen | 51304 | |
| Commercial assay or kit |
RNeasy Mini kit | Qiagen | 74104 | |
| Gene | QKI | NCBI Reference | NM_006775.2 | |
| Gene | RBFOX1 | NCBI Reference | NM_145893.2 | |
| Strain, strain background |
NCr-nude mice | Taconic | NCRNU,F, CrTac:NCr,Foxn1nu |