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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Cytokine. 2018 May 18;110:420–427. doi: 10.1016/j.cyto.2018.05.012

Figure 4. Gross anatomy, histology, and IL-33 immunohistochemistry for C Kit (−/−) and wild type mice after LL-37 challenge.

Figure 4

A) Examination of the gross anatomy of bladders 24 hours after LL-37 challenge demonstrated increasing inflammation within the inner mucosa for both C-kit (−/−) and wild type mice in a dose response fashion. Bladders were hemisected to allow for inspection of the urothelium. Scale bar represents 5 mm. B) Histological examination demonstrated more pronounced inflammation in the wild type mice compared to the C-kit (−/−) group. Edema, polymorphonuclear cell infiltrate, and microabscesses were more pronounced in the normal mast cell intact mice compared to the C-kit (−/−) group. Scale Bar represents 250 μm. C) Immunohistochemistry demonstrated increasing tryptase expression, which is indicatory of mast cells, in the lamina propria and urothelium as exposure to concentrations of LL-37 increased in only the WT mice. No tryptase expression was observed in the C-kit (−/−) mice at all concentrations of LL-37 challenge. Scale bar represents 100 μm. LU: lumen of the bladder. U: urothelium. SM: submucosa. LP: lamina propria. BSM: bladder smooth muscle layer.