Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Immunol. 2018 Jul 16;201(5):1536–1548. doi: 10.4049/jimmunol.1701240

Figure 2. Ectopic expression of 2B4 results in reduced accumulation of antigen-specific CD8+ T cells but does not impact function of antigen-specific CD8+ T cells on a per cell basis.

Figure 2

A, Representative flow cytometry plots gated on CD8+ cells isolated from the spleen. B, Frequencies and absolute numbers of CD44hi Thy1.1+ of CD8+ cells. C, IFN-γ+ pMY or 2B4rg cells following ex vivo stimulation with PMA and ionomycin. D, Summary of the frequency of IFN-γ-producing Thy1.1+ CD8+ T cells, representative of 2 independent experiments with 7-8 mice/group. E, Summary of the absolute numbers of IFN-γ-producing Thy1.1+ CD8+ T cells, representative of 2-3 independent experiments with 7-12 mice/group. *p<0.05, ** p<0.001, *** p<0.0001.