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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Cytokine. 2018 May 17;110:213–221. doi: 10.1016/j.cyto.2018.05.005

Figure 3. MyD88, IRAK4 and NF-kB expression by peripheral blood CD14+ monocytes of HIV− and HIV+ individuals with latent tuberculosis infection and active tuberculosis disease.

Figure 3

CD14+ monocytes from PBMC of HIV+LTBI+, HIV−LTBI+ individuals, HIV+TB and HIV−TB patients (8 donors in each group) were isolated by magnetic selection and cultured with or without γ-irradiated Mtb H37Rv (10 μg/ml) or TLR2 agonist Pam3CSK4 (10 μg/ml). After 48 hrs, MyD88 and IRAK4 expression was determined by PCR analysis. Boxes show the median and interquartile range, and whiskers show the 5th and 95th percentile values. A. γ-irradiated Mtb H37Rv stimulated cells. B. TLR2 agonist Pam3CSK4 stimulated cells.