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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Mammary Gland Biol Neoplasia. 2018 Jul 3;23(3):165–176. doi: 10.1007/s10911-018-9402-6

Fig. 3. EV secretion is required for the establishment of epithelial polarity.

Fig. 3

(a) Western blot showing indicated protein levels in MCF10A control and RAB27 KD cells. (b) Numbers of EVs secreted by equal number of indicated cells were determined by nanoparticle tracking analysis. *** P < 0.001 compared to the MCF10A control cells. (c) MCF10A (control or RAB27 KD), MCFDCIS, and HuMEC cells were seeded on 24-well transwell inserts and TEER was measured over the indicated time course. *** P < 0.001 compared to the MCF10A control cells. (d) Left: Phase contrast images of indicated cells grown on tissue culture dishes. Right: The XZ-view of reconstructed Z-stack images showing the immunofluorescence staining of Podocalyxin (green) and Syntaxin-4 (red) in cells grown on filters. DAPI staining (blue) indicates the nuclei. Green and red arrows indicate apical staining of Podocalyxin and basolateral staining of Syntaxin-4, respectively.