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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Int J Cancer. 2018 Apr 16;143(5):1188–1201. doi: 10.1002/ijc.31405

Figure 3. EphA2 knockdown reduces ES cell viability.

Figure 3

(A) Representative western blot showing EphA2 expression in silencing models generated from A673 and TC252 cell lines. (B) Proliferation assay using the WST1 tetrazolium-based assay. (C) Quantification of clonogenic assay. Representative images are shown above the bars. “Clone A” and “B” denote clones AshE3 and TshE26 and AshE15 and TshE35, respectively. (D) In vivo tumor growth in immunodepressed mice after subcutaneous injection of cells. Tumor size at the time of euthanasia is shown; n=8. SCR = control scrambled sequence. AshE#/TshE# = EphA2 silenced selected clones. Data are presented as means ± SD. Statistical significance was achieved by the Student’s t test from at least three different experiments: *p ≤ 0.05 **p ≤ 0.01.