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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Int J Cancer. 2018 Apr 16;143(5):1188–1201. doi: 10.1002/ijc.31405

Figure 4. EphA2 knockdown reduces ES cells motility.

Figure 4

(A) Migration assay in Boyden chambers using EphA2 silenced models. “Clone A” and “B” denote clones AshE3 and TshE26 and AshE15 and TshE35, respectively. SCR models were set as reference. (B) Invasion assay in Matrigel-coated Boyden chambers using EphA2 silenced models. “Clone A” and “B” denote clones AshE3 and TshE26 and AshE15 and TshE35, respectively. SCR models were set as reference. (C) Lung metastasis incidence in immunodepressed mice after tail-vein injection of control or EphA2 silenced A673 cells; n = 10. (D) Lung metastasis incidence in immunodepressed mice after injection of tumor cells in the gastrocnemius; n = 7. SCR = control scrambled sequence. Data are presented as means ± SD. Statistical significance was achieved by the Student’s t test from at least three different experiments. Fisher’s exact test was used for evaluating differences in lung metastasis incidence in mice: *p ≤ 0.05 **p ≤ 0.01.