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(a) Liquid chromatography–mass spectrometry (LC–MS) analysis of 2-HEP-CMP (black), [2-2H2]-2-HEP-CMP (red), (2R)-[2-2H1]-2-HEP-CMP (green), and (2S)-[2-2H1]-2-HEP-CMP (blue). (b) LC–MS analysis of 2-HPP-CMP (left) and 5′-dA (right) produced from SUMO-Fom3 reactions with 2-HEP-CMP isotopologues.
