B cell defects in Nod2−/− mice are due to a mutation in Dock2 and independent of Nod2 deficiency. (A) Breeding scheme to generate WT and Nod2−/− littermate controls. (B) Representative plot showing percentages of marginal zone (CD21 high CD23 low) B cells in F2 generation littermates. (C) PCR for presence of Dock2mut in WT, Nod2−/−, Nod2−/− Jax, Rip2−/−, and F2 Nod2−/− mice. (D) Summary table showing number of mice with normal B cell populations as indicated/total number of mice tested. (E) PCR for presence of Dock2mut in WT, Jax Cd11a−/− mice, and Nod2−/−Dock2mut mice. Data is representative of three independent experiments.