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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Chem Phys Lipids. 2018 Jul 22;215:18–28. doi: 10.1016/j.chemphyslip.2018.06.002

Figure 3: Tubulation is driven by expansion of the SLB.

Figure 3:

(A-C) Images of a scratched SLB at various time points during exchange of the bathing solution by perfusion from water into Buffer A. Further expansion of the bilayer beyond that which was necessary to fill the scratched region results in tubule formation. Scale bars, 10 µm. (D) Changes in average fluorescence intensity per unit area upon exchange of the bathing solution from Buffer A into water (gray) or vice versa (black). SLB retraction is indicated by an increase in fluorescence (2.7 ± 0.3 percent increase exchanging from Buffer A into water). Expansion is indicated by decreased fluorescence (4.9 ± 0.9 percent decrease exchanging from water into Buffer A). All SLBs were 3:1 DOPC/DOPS with 0.1% LRB-DOPE. Error bars are ± SEM.