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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Chem Phys Lipids. 2018 Jul 22;215:18–28. doi: 10.1016/j.chemphyslip.2018.06.002

Figure 4: Sar1B membrane remodeling.

Figure 4:

The addition of Sar1BGTP to STuBs alters the morphology of lipid tubules. (A) A flexible tubule. (B) Same tubule as in (A) following addition of Sar1BGTP, exhibiting a rigid morphology. (C) A pseudo-vesiculated tubule. (D) A rigid, bifurcated tubule and a tubule exhibiting a beads-on-a-string morphology. (E) As Sar1BGTP concentration increases, the number of flexible tubules per field of view significantly decreases. (F) The prevalence of Sar1BGTP-induced structures changes as a function of concentration. Statistics by 1-way ANOVA. * indicates comparison to 0-nM Sar condition, † indicates comparison to 50-nM Sar1B condition. Adjusted P Values; *, †: P < 0.05, **, †† P: < 0.01, ***, †††: P < 0.001. All are in Buffer A + 0.5 mM MgCl2 + 5 μM GTP. Scale bars 10 µm. Error bars ± SEM.