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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Chem Phys Lipids. 2018 Jul 22;215:18–28. doi: 10.1016/j.chemphyslip.2018.06.002

Figure 7: Sar1B bends membranes in a GTP-dependent manner.

Figure 7:

(A) Increases in DiD-labeled membrane curvature (P/S) and concentration (P + 2S) observed after 10 nM Sar1BGTP is added to STuBs. (B-C) Graphs corresponding to the event shown in panel A. Averaged P/S (D) and P+2S (E) changes for several membrane budding events observed in the presence of Sar1BGTP (n=5). Error bars ± SEM. No similar events were observed in otherwise identical samples with Sar1BGDP or lacking protein (n ≥ 3). (G) Predicted structure of the detected membrane indentations based on computer simulations (Anantharam et al., 2010). Scale bars 2 µm.