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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Chem Phys Lipids. 2018 Jul 22;215:18–28. doi: 10.1016/j.chemphyslip.2018.06.002

Figure 8: Sar1B drives tubule fission in a GTP-dependent manner.

Figure 8:

Effect of wild-type Sar1B on tubule density in the presence of 5 µM GDP, 5 µM GTP, and 5 µM GMP-PNP. In these experiments, 50 nM Sar1B was titrated onto an SLB containing approximately 100 pre-formed tubules in each field of view sampled. *:P < 0.05, **: P < 0.01, ***:P < 0.001, ****:P < 0.0001 relative to the absence of protein. Error bars ± SEM. To test for significance, data were compared to 0 nM condition using Student’s t-test. All samples used 3:1 DOPC/DOPS with 0.1% LRB-DOPE as the fluorescent tracer.