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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Chem Phys Lipids. 2018 Jul 22;215:18–28. doi: 10.1016/j.chemphyslip.2018.06.002

Figure 9: Model for STuBs formation and effects.

Figure 9:

(1) SLB deposition occurs via liposome fusion. In the presence of high salt, liposomes adsorb to the glass surface faster than they rupture and spread, resulting in a bilayer containing excess lipid. (2) Washing with water removes unincorporated liposomes; although the bilayer still contains excess lipid, it appears flat for reasons that could involve decreased area per lipid and/or stronger lipid-glass interaction in water (see text). (3) Following addition of physiological ionic strength buffer, the excess lipid is no longer accommodated in the planar bilayer and tubules form. (4a-b) Effects of Sar1B addition with various nucleotides.