Skip to main content
. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: J Immunol. 2018 Jul 18;201(5):1382–1388. doi: 10.4049/jimmunol.1800585

FIGURE 5.

FIGURE 5

Assessment of the fasting and refeeding effects on epithelial airway cell inflammation and the Th2 endotype. (A) IL-1β release from fasted or refed steroid-naïve PBMCs co-cultured with BEAS-2B lung epithelial cells incubated in their respective serum in the presence of HDM extract (n=5). (B-C) IL-17E and TSLP release from BEAS-2B epithelial cells co-cultured with PBMCs (n=5). (D) mRNA expression of genes encoding NLRP3 and GATA3 from negatively selected CD4+ T-cells from steroid-naïve PBMCs (E, n=10). (E) Representative protein levels of GATA3 in CD4+ T-cells. (F) Quantification of GATA3 protein levels comparing fasted to refed states in steroid-naïve subjects (n=8). (G) IFNγ, IL-4, and IL-17A cytokine release as measured by ELISA following the stimulation of fasted and refed PBMCs with PMA and ionomycin (n=5) Statistical analysis using paired student t-test with p values shown in each panel.