Immunoblot analysis of β-glucuronidase (GUS) expression in the vascular tissues of transgenic tobacco plants: In lines 35S, uidA is regulated by the cauliflower mosaic virus 35S promoter; in lines Z4, uidA is regulated by the promoter of Arabidopsis thaliana XYLOGEN PROTEIN 1; in lines Z5, uidA is regulated by the chimeric promoter 35S-Px.M, protein markers; W, wild type tobacco (negative control). The endogenous actin protein was detected using an actin-specific antibody and used as a loading control