Fig. 1.

Apigenin (API) inhibits colony formation, cell migratory, and invasive abilities of non-small cell lung cancer (NSCLC) cells harboring different epidermal growth factor receptor (EGFR) statuses. a Four NSCLC cell lines, A549 and CL1–5 (wild-type EGFR), HCC827 (del E746-A750), and H1975 (L858R/T790 M), were treated with the indicated concentrations of API for 24 or 48 h, and cell viability was determined with an MTS assay. b A549 and HCC827 cells were treated with the vehicle or API (5~40 μM) for 24 h; then, the death-inducing effects of API on cells were determined by counting the colonies formed. c, d Four NSCLC cell lines (A549, CL1–5, HCC827, and H1975) were treated with indicated concentrations of API for the transwell-migration (c) and Matrigel-invasion (d) assays. b-d Left: Representative photomicrographs (100×). Scare bar, 500 μm. Right: Data are presented as the mean ± SD of at three independent experiments. * p < 0.05, compared to the vehicle group