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. 2018 Aug 21;8:12519. doi: 10.1038/s41598-018-31089-y

Figure 2.

Figure 2

Co-assembled nanofibers bearing Mtb-specific CD8+ T cell epitopes elicit antigen specific immune responses. Schematic shows self-assembly of single or dual epitope nanofiber formulations (A). Gating strategy used for identification of activated CD8+ T cells isolated from draining lymph nodes 30 days post-immunization (B). Representative flow plots for IFN-γ+ CD8+ T cells in mice vaccinated with single epitope or dual epitope formulations (C). Mean percentages ± SEM of IFN-γ+ CD8+ T cells (D), and IL-2+ CD8+ T cells (E) measured ex vivo in lymph nodes of mice vaccinated with TB10.4 or ESAT6 nanofibers or co-assembled nanofibers. Data is representative of two independent experiments with similar results and ***p < 0.001, **p < 0.01 by one-way ANOVA with multiple comparisons of means.