Table 1.

IC₅₀ for inhibition of ceramide synthases by P053

	hCerS1	mCerS1	hCerS2	mCerS2	hCerS4	mCerS5	hCerS6
P053 [2]	0.54 ± 0.06	0.46 ± 0.08	28.6 ± 0.15	18.5 ± 0.12	17.2 ± 0.09	7.2 ± 0.10	11.4 ± 0.17
G024 [1]	6.0 ± 0.10		14.5 ± 0.09		18.0 ± 0.12	24.3 ± 0.22	8.5 ± 0.13
FTY720	9.9 ± 0.07	5.6 ± 0.05	19.1 ± 0.10	10.6 ± 0.13	26.0 ± 0.18	15.0 ± 0.07	5.8 ± 0.20
FB1	0.22 ± 0.05		0.15 ± 0.08		0.92 ± 0.06	0.50 ± 0.05	0.29 ± 0.05

Calculated IC₅₀ values (μM) for inhibition of different human (h) or murine (m) CerS isoforms by P053, G024, FTY720, and FB1. IC₅₀ values were calculated using an inhibitor range from 10 nM–100 μM, as shown in Fig. 1b