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. Author manuscript; available in PMC: 2019 Aug 16.
Published in final edited form as: Mol Cell. 2018 Aug 16;71(4):567–580.e4. doi: 10.1016/j.molcel.2018.06.039

Figure 1. LYR proteins require ACP to execute their functions as complex-specific ETC assembly factors.

Figure 1

A. A structural model depicting the interaction between ACP (blue) and ISD11 (LYR; green). The 4′-PP and a bound acyl chain are colored by element. PDB ID: 5USR (Cory et al., 2017). B. Mitochondrial lysates generated from the indicated strains harboring the indicated plasmids were separated by BN-PAGE and immunoblotted with the indicated antibodies (EV – empty vector). C-F. Acp1HA/FLAG or Acp1S82A-HA/FLAG were immunoprecipitated from cells expressing Sdh7V5 (C,D) or Mzm1V5 (E,F). The inputs and eluates were immunoblotted with the indicated antibodies. G,H. Acp1HA/FLAG was immunprecipitated from WT, mct1Δ, and oar1Δ cells expressing Mzm1V5 (G) or Sdh7V5 (H). The inputs and eluates were immunoblotted with the indicated antibodies. Inputs represent 5% of the total lysate.