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. 2018 Aug 21;8:12522. doi: 10.1038/s41598-018-30978-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Cell growth of ovarian cancer cells A2780 and SK-OV-3 was measured, in the presence of increasing concentrations of resistin, by MTT assay after 72 hours of resistin exposure. The OD value for control cells (0 ng/mL Resistin) was assigned a value of ‘1’ and the relative OD values of resistin-treated cells are plotted to represent cell growth index. (B) A time-dependent induction of cell growth was measured by incubating A2780 and SK-OV-3 cells with 25 ng/mL resistin for 24, 48 and 72 hours. The OD value for control cells (0 hours) was assigned a value of ‘1’ and the relative OD values at 24, 48 and 72 hour time-points are plotted to represent cell growth index. Anchorage-dependent colony formation assay (C) and invasion assays (D) were performed to assess the effects of 25 ng/mL resistin on colony forming ability and invasive potential of A2780 and SK-OV-3, when incubated for 72 hours. For invasion assay, the number of cells that invaded through matrigel were calculated after staining with Calcein AM (left graph) and the fluorescence of collected cells quantitated (right graph). Representative pictures for colonies and invasive cells are shown below the respective bar graphs. ‘+Res’ in representative pictures indicates assay in the presence of added resistin. *p < 0.01, compared to control and ^#p < 0.01, compared to cell growth at 24 h.