Figure 5.

WIN mitigates the connexin 43 (Cx43) hemichannel-dependent release of ATP evoked by high glucose and IL-1β/TNF-α in endothelial cells. Averaged data of ATP release from EAhy cells treated for 72 h with 5 mM glucose (control, white bar), 25 mM glucose, and IL-1β/TNF-α (black bars) alone or in combination with the following agents: 100 µM gap19, 100 µM Tat-L2, 100 µM ¹⁰panx1, 500 µM probenecid (Prob), 5 µM, WIN and/or 5 µM SR-141716A (SR1). *p < 0.05, IL-1β/TNF-α and high glucose compared to control; ^#p < 0.05, effect of each agent compared to the effect induced by IL-1β/TNF-α and high glucose; ^&p < 0.05, effect of each cannabinoid receptor antagonist compared to the effect of the respective cannabinoid (one-way analysis of variance followed by Tukey’s post hoc test). Data were obtained from at least three independent experiments (see scatter dot plot) with four repeats each one.