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3D imaging of adult mouse brains cleared with a-uDISCO (Thy1-GFP-M). (A) 3D reconstruction of neuronal structures in the whole brain. (B–F) High-magnification MIP images of the boxed regions indicated in (A) demonstrating that neuronal structures could be visualized at a subcellular resolution in the cortex (B), hippocampus (C), corpus striatum (D), superior colliculus (E), and cerebellum (F). The arrow indicates fine branches of neurons.
