FIGURE 5.

PH-BEACH domain of Nbea is sufficient to rescue the defect on mushroom spines. (A) Representative dendrites of primary hippocampal neurons from wild-type (WT) and Nbea null-mutant (KO) mice transfected with cytosolic marker t-dimer-RFP alone and in combination with GFP-tagged full-length Nbea (KO + Nbea FL) or PH-BEACH domain (KO + PH-BEACH). As additional control, KO neurons were mock transfected with GFP alone (KO + GFP). Scale bar: 2.5 μm. (B) Quantification of the number of mature mushroom-like DSs in different genotypes and upon expression of different constructs as indicated. Data are means ± SEM; N, number of neurons (in bars). Quantification was carried out analyzing the whole dendritic tree for each neuron. ^∗P < 0.05 ^∗∗∗P < 0.001, n.s., not significant by one-way ANOVA with Tukey’s multiple comparisons test.