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. 2018 Aug 15;9:1739. doi: 10.3389/fmicb.2018.01739

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Copyright © 2018 Smith, Kaczmar, Bamford, Smith, Frustaci, Kovacs-Simon, O’Neill, Moore, Paszkiewicz, Titball and Pagliara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth phase dependence of the fraction of persisters to gentamicin, ofloxacin, or ampicillin. Temporal dependence of the normalized fraction of persisters to treatment either with gentamicin (A), ofloxacin (C), or ampicillin (E), with the addition of fresh LB, or with antibiotics only [(B,D,F), respectively]. At t = 0 an overnight E. coli culture was diluted 1:1000 in LB medium and the culture growth started. Each data set is normalized to its maximum persister fraction. Data and error bars are the mean and SEM of measurements obtained at least on biological and technical triplicates.